Journal: PLoS ONE

Article Title: Dietary Restriction Affects Neuronal Response Property and GABA Synthesis in the Primary Visual Cortex

doi: 10.1371/journal.pone.0149004

Figure Lengend Snippet: The top panel shows a typical sample of a Western blot result from each DR cat (DR1-4) and control cat (NC1-4) using GAD67 and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) antibodies. The bottom panel shows the average optical density (OD) of GAD67 bands normalized against the corresponding GAPDH in both groups of cats. The average normalized optical density of GAD67 in DR cats was significantly higher than that in the control (** p<0.01).

Article Snippet: The membrane was blocked with 5% non-fat dry milk in TBS-Tween 20 for one hour and incubated overnight at 4°C in TBS-Tween 20 containing a rabbit polyclonal antibody GAD67 (dilution 1:200; Boster Bio-engineering Limited Company) or a rabbit polyclonal antibody GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (dilution 1:5,000; Sangon Biotechnology).

Techniques: Western Blot, Control

Journal: Cells

Article Title: TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression

doi: 10.3390/cells10102751

Figure Lengend Snippet: Cellular identification of GFP-expressing cells of the DG, LEC, and Cb in P56 of TWIK-1 BAC-GFP Tg mice. ( A ) Overview of GFP expression in DG, LEC, and CB of TWIK-1 BAC-GFP Tg mice. Scale bar, 200 μm. ( B ) Representative co-immunofluorescence images with GFP, NeuN, and GAD67 antibodies. Scale bar, 200 μm. ( C ) Quantification bar graph of the cell type of GFP-positive cells in each brain area from B. Quantification was analyzed by the percentage of each cell type from all GFP-positive cells. Raw data are listed in . Data are presented as the Mean ± SEM.

Article Snippet: The following antibodies were used: chicken anti-GFP (Abcam, Cat#; ab136970, 1:300); rabbit anti-TWIK-1 (Alomone labs, Cat#; APC-10, 1:200); mouse anti-NeuN (Abcam, Cat#; ab104224, 1:100); rabbit anti-GAD67 (GeneTex, Cat#; GTX113190, 1:300); guinea pig anti-doublecortin (Millipore, Cat#; AB2253, 1:100); rabbit anti-calbindin (Swant, Cat#; CB38, 1:2000); rat anti-Ki67 (Invitrogen, Cat#; 14-5698-85, 1:200); rat anti-GFAP (Invitrogen, Cat#; 13-0300, 1:500); rabbit anti-NG2 (Millipore, Cat#; AB5320, 1:300); and Alexa Fluor 488-, 594-, and 647-conjugated secondary antibodies (Jackson ImmunoResearch, 1:300).

Techniques: Expressing, Immunofluorescence